HPLC Columns: The Backbone of High-Performance Liquid Chromatography
HPLC Columns – Introduction HPLC columns are the core component of High-Performance Liquid Chromatography (HPLC), playing a vital role in separating, identifying, and quantifying components in complex mixtures. The performance and reliability of your HPLC system depend heavily on the column you choose. Whether you're analyzing pharmaceuticals, biomolecules, food contaminants, or environmental samples, the right HPLC column ensures high resolution, accuracy, and repeatability. In this article, we’ll explore everything you need to know about HPLC columns —from their types and functions to how to choose the best column for your needs. What Are HPLC Columns? HPLC columns are tubular devices packed with stationary phase materials, typically silica or polymer particles, that interact with analytes as they pass through with a mobile phase. The separation mechanism is based on differences in the analytes’ affinities for the stationary phase. Every HPLC column is designed to meet ...